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ISCRM

Mathematics and Science Center
118 Library Drive
Rochester, MI 48309-4479
(location map)

ISCRM

Mathematics and Science Center
118 Library Drive
Rochester, MI 48309-4479
(location map)

A woman seated at a desk, looking at magnified biological samples on a computer screen.

Research and Publications

Whether it’s stem cell biology, cancer, tissue bioengineering, regenerative medicine or therapeutics: The ISCRM promotes research involving molecular mechanisms that maintain stemness and induce differentiation in stem cells (SCs). Stem cells provide a renewable resource for tissue engineering and cell therapy. Embryonic stem cells (ESCs) have the greatest potential to self-renew and capacity to differentiate. Cord blood stem cells (CBSCs) and adult stem cells (ASCs) are less potent but are more desirable sources for therapeutic applications. Curious to learn more? Below are potential areas of stem cell research.

Research Programs

Regulation of stemness and differentiation of ESCs, CBSCs and ASCs

  • Neurogenesis
  • Osteogenesis
  • Chondrogenesis
  • Adipogenesis
  • Myogenesis

Umbilical cord stem cells

  • Future treatments for developmental and early childhood diseases, including Leukemia, Sickle-cell anemia, and various immunodeficiencies
  • Use of CBSCs to investigate inherited disorders and disease processes

Induced pluripotent stem (iPS) cell generation, stability, and differentiation

  • Normal skin cells
  • Reverse engineering disease pathways

Tissue engineering using human stem cells

  • Bone and cartilage tissues/structures
  • Nucleus pulposis in degenerated intervertebral disc
  • Neural tissues damaged by spinal cord injuries
  • Fabrication of scaffolds and devices for stem cell collection and tissue regeneration

Restoration of damaged tissue/organ function

  • Regeneration of pelvic nerve to restore detrusor muscle function in SCIs
  • Treatment of retinal degeneration using stem cell therapy
  • Cardiovascular injury and repair
  • Stem cells for wound healing

Treatment of developmental and age-related degenerative diseases using stem cell therapy

  • Leukemia
  • Incontinence
  • Multiple sclerosis
  • Parkinson’s disease
  • Alzheimer’s disease
  • Diabetes
  • ALS

Drug discovery and development

  • miRNA based drugs

Toxicological studies

  • Development of the NET (New Embryotoxicity) assay for screening drugs and chemicals
  • Mechanisms of DNA damage and repair in SCs
Research Projects

Dr. Chaudhry studies a wide-range of basic and applied biomedical research problems. His research is focused on:

Embryonic Stem Cell (ESCs), Adult Stem cells (ASCs), Perinatal stem cells (PSCs) and Induced Pluripotent Stem Cells (IPSCs); cellular development and differentiation; Tissue engineering and regeneration; Biotechnology and toxicology.

  1. Collection and characterization of umbilical cord blood stem cells for potential therapeutic uses
  2. Intervertebral disc regeneration using cord blood derived stem cells
  3. Role of mononuclear cells in repair and regeneration
  4. Self-assembling scaffolds for embryonic stem cell (ESC) culture, storage and shipping
  5. Stem cell therapy for treatment of macular degeneration
  6. Effect of estrogen and progesterone on embryonic cell development and differentiation
  7. Potential and feasibility of stem cell therapy for treatment of Parkinson’s disease
  8. Neural differentiation of stem cells into oligodendrocytes for treatment of multiple sclerosis (MS)
  9. Effect of heavy metals on the developmental and differentiation processes 
  10. Dopaminergic differentiation of cord blood derived stem cells
  11. Differentiation of ESCs into islet cells; potential cell therapy for the treatment of diabetes mellitus
  12. Bone and cartilage tissue bioengineering for TMJ reconstruction
  13. Stem cells for wound healing
  14. Pharmacological and toxicological studies using ESCs as a model for early development and differentiation
  15. Human chip for pharmacological and drug development studies
  16. Peripheral and cord blood derived HSC propagation for cell therapy
  17. Neural mapping of brain and nerve regeneration using ESCs derivatives
  18. iPSCs for reverse engineering disease pathways

Publications

McKee C, Brown C, Bakshi S, Walker K, Govind C, Chaudhry GR. 2021. Transcriptomic Analysis of Naïve Human Embryonic Stem Cells Cultured in Three-Dimensional PEG Scaffolds. Biomolecules. 11(1): 21.

Nour S, Imani R, Chaudhry GR, Sharifi AM. 2020. Skin wound healing assisted by angiogenic targeted tissue engineering: A comprehensive review of bioengineered approaches. Journal of Biomedical Materials Research Part A. doi: 10.1002/jbm.a.37105.

McKee C, Brown C, Chaudhry GR. 2019. Self-assembling scaffolds supported long-term growth of human primed embryonic stem cells and upregulated core and naïve pluripotent markers. Cells. 8(12): 1650.

McKee C, Beeravolu N, Brown C, Perez-Cruet M, Chaudhry GR. 2019. Mesenchymal stem cells transplanted with self-assembling scaffolds differentiated to regenerate nucleus pulposus in an ex vivo model of degenerative disc disease. Applied Materials Today. 100474.

Brown C, Mckee C, Bakshi S, Walker K, Hakman E, Halassy S, Svinarich D, Dodds R, Govind C, Chaudhry GR. 2019. Mesenchymal Stem Cells: Sources, Therapeutic Potential, and Challenges. doi: 10.1002/term.2914.

Bakshi S, Mckee C, Walker K, Brown C, Chaudhry GR. 2018. Toxicity of JQ1 in neuronal derivatives of human umbilical cord mesenchymal stem cell. Oncotarget. 9 (73):33853-33864. doi:10.18632/oncotarget.26127.

McKee C, Beeravolu N, Bakshi S, Thibodeau B, Wilson G, Perez-Cruet M, Chaudhry GR. 2018. Cytotoxicity of radiocontrast dyes in human umbilical cord mesenchymal stem cells. Toxicology and Applied Pharmacology. 349: 72-82. doi: 10.1016/j.taap.2018.04.032.

Perez-Cruet M, Beeravolu N, McKee C, Brougham J, Khan I, Bakshi S, Chaudhry GR. 2018. Potential of human NP-like cells derived from umbilical cord to treat degenerative disc disease. Neurosurgery. doi: 10.1093/neuros/nyy012.

McKee C, Chaudhry GR. 2017. Advances and challenges in stem cell culture. Colloids and Surfaces B: Biointerfaces. 159: 62-77. doi.org/10.1016/j.colsurfb.2017.07.051.

McKee C, Yao D, Chaudhry GR. 2017. Chondrogenic differentiation of embryonic stem cells using mechanotransductive 3-D PDMS scaffolds. Stem Cell and Transnational Investigation. 4:e1536. doi: 10.14800/scti.1536.

Beeravolu, N, C. McKee, G.R. Chaudhry. 2017. Mechanism of arsenite toxicity in embryonic stem cells. Journal of Applied Toxicology.  37(10): 1151-1161. doi: 10.1002/jat.3469.

McKee, C, Hong, Y, Yao, D, Chaudhry, GR. 2017. Compression induced chondrogenic differentiation of embryonic stem cells in 3-D PDMS scaffolds. Tissue Engineering Part A. 23(9-10): 426-435. doi: 10.1089/ten.TEA.2016.0376.

Beeravolu N, Brougham J, Khan I, McKee C, Perez-Cruet M, Chaudhry GR. 2017. Human Umbilical Cord Derivatives Regenerate Intervertebral Disc. Journal of Tissue Engineering and Regenerative Medicine. 12(1): 579-591. doi: 10.1002/term.2330.

Beeravolu N, McKee C, Alamri A, Mikhael S, Brown C, Perez-Cruet M, Chaudhry GR. 2017. Isolation and characterization of mesenchymal stem cells from different regions of the human umbilical cord. Journal of Visualized Experiments. (122): e55224. doi: 10.3791/55224.

Mikhael S, Beeravolu N, Chaudhry GR. 2016. Umbilical cord derivatives for intervertebral disc regeneration: advances and challenges. Editorial. Cell and Gene Therapy Insights. 2(6): 629-634. doi: 10.18609/cgti.2016.076.

Beeravolu N, Khan I, McKee C, Dinda S, Thibodeau B, Wilson G, Perez-Cruet M, Bahado-Singh R, Chaudhry GR. 2016. Isolation and comparative analysis of potential stem cells from different regions of human umbilical cord. Stem Cell Research. 16: 696-711. doi: 10.1016/j.scr.2016.04.010.

Alghamdi S, Khan I, Beeravolu N, Mckee C, Thibodeau B, Wilson G, Chaudhry GR. 2016. BET protein inhibitor JQ1 inhibits growth and modulates WNT signaling in mesenchymal stem cells. Stem Cell Research and Therapy. 7:22. doi: 10.1186/s13287-016-0278-3.