Cell and Molecular Biology

Cell and Molecular Biology

Cell and Molecular Biology Support Module
 
Purpose:
 
The module provides central facilities and expertise for cell and tissue culture, cryopreservation of cell lines, mycoplasma test monitoring of cell lines, DNA sequencing, guidance for PCR design and DNA construct design, real time PCR for genotyping and gene expression assays (Sybr-green and Taqman chemistries, mouse and human), as well as basic training and use of Bioinformatics tools (stand alone and web based). Management of ChIP-on-Chip (Chromatin Immunoprecipitation on DNA Chip array) project design and delivery is also possible to explore RNA-Polymerase-II binding, Transcription Factor binding, and Epigenomic regulation of human and mouse genes. (26,000 genes) 
 
The module recently supported development of the NEIBank gene expression database, for Guinea pig ocular tissues: Lens, Retina, and Rest of Eye (minus lens and retina). This project represents a collaboration of the ERI (Oakland University), the NEI (National Eye Institute, National Institutes of Health), and the Oregon Health Sciences University. Over 4000 expressed sequence tags (ESTs) for each tissue library were submitted to GenBank, and support the annotation of the first draft of the Guinea pig genome (2008). NEIBank is a free resource, funded by the NIH, to support vision researchers world wide. Unlike the mouse, rat, and many other mammals, there was little EST data for any Guinea pig tissue prior to this project. The EST sequences are not only valuable to vision scientists, they are essential for determining gene structures (introns, exons, alternate splicing) within the Guinea pig genomic sequence (3 billion bases of DNA long). This genome is now available, along with the human, mouse, and other mammalian genomes, in the Genome Browser database at UCSC:      http://genome.ucsc.edu/  
 
The peer-reviewed publication describing this new resource is also free at the journal Molecular Vision:   http://www.molvis.org/molvis/v14/a278/  
 
NEIBank can be accessed at:    http://neibank.nei.nih.gov/
 
 
Equipment:
 
Tissue culture hoods are available for BioSafety Level -2 work (BSL-2).
 
A wide screen Unix (Mac OS-X Server) work station is available for molecular biology and bioinformatics projects with multiple protected accounts for many labs and users.
 
Locations of Mol & Cell Biology services and equipement:
 
Rm 427 (Tissue Culture, Autoclave, Water purification)
 
Rm 429 (Ultracentrifuge, bench Autoclave, Vacu-fuge, Mastercycler gradient, Biospec, UV-Stratalinker)
 
Rm 430 (Real time PCR, Automated DNA sequencing)
 
Rm 431 (Molecular workstation, student computer access, work room, MacVector, Osprey Software.)
 
Rm 414 (in situ hybridization, immunochemistry)
 
 
User Access and Biosafety Compliance:
 
All users of any part of the module must obtain, read, and sign for a copy of the Module's Standard Operating Procedures for Biosafety. A copy may be obtained from Dr. Kenneth Mitton, and is on the desk just inside Room 427.
 

Dr. Kenneth Mitton
Associate Professor of Biomedical Sciences, and
Director Cell and Molecular Biology support module
412 Dodge Hall of Engineering
(248) 370-2079